Biotin-KRAS[G12D/Y64H]
KRAS2,RASK2
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Catalog Number:E2505T-H01HA
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human Biotin-KRAS[G12D/Y64H] protein with N-terminal His tag and C- terminal Avi tag was purified by Ni-NTA affinity and specifically biotinylated at Avi tag in vitro, followed by SEC chromatography. The Biotin-KRAS[G12D/Y64H] protein showed high activity in HTRF assay.
Species：Human
Tag：N-terminal-6x His tag-TEV, C- terminal Avi tag
Expression Host：HEP E.coli
Uniprot：P01116-2
Sequences：M1-K169
Molecular Weight：23.3 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
![M150612021-Biotin-KRAS[G12D Y64H].png](https://protein.ice-biosci.com/uploads/20251027/26c96130ba54d0d2.png)
Activity Identification：The Biotin-KRAS[G12D/Y64H] activity was detected using HTRF technology. The reaction was performed by incubating the Biotin-KRAS[G12D/Y64H] protein, substrate and beads at 25℃ for 60 min, then reading ratio 665/520 with BMG.
![M150612021-Biotin-KRAS[G12D Y64H].png-1.](https://protein.ice-biosci.com/uploads/20251027/88d2fdec4543950d.png)
![M150612021-Biotin-KRAS[G12D Y64H].png-2.](https://protein.ice-biosci.com/uploads/20251027/74c277d23d174ca0.png)
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
