Biotin-KRAS[G12A]
Biotin-KRAS[G12A]
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Catalog Number:E2402T-H02HA
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Description：Recombinant human Biotin-Kras[G12A] protein with N-terminal 8 x His tag and C-terminal Avi tag was purified by Ni-NTA affinity, biotinylation and followed by SEC chromatography. The Biotin-Kras[G12A] protein showed high activity in HTRF assay.
Species：Human
Tag：N-terminal 8x His tag, C-terminal Avi tag
Expression Host：HEP E.coli
Uniprot： P01116-2
Sequences：M1-K169
Molecular Weight：23.6 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-KRAS[G12A] activity was detected using HTRF technology. The reaction was performed by incubating the Biotin-KRAS[G12A]protein, GTP and SOS1 at 25℃ for 60 min, adding cRAF at 25℃ for 15 min and adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
