KRAS[G12D]
GTPase KRas, KRAS2, RASK2
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Catalog Number:E2308F-H04H
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COA:Please contact us
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Description：Recombinant human KRAS[G12D] protein with N-terminal 6x His tag was purified by Ni-NTA affinity and followed by SEC chromatography. The KRAS[G12D] protein showed high activity in HTRF assay.
Species：Human
Tag：N-terminal 6x His tag
Expression Host：HEP E.coli
Uniprot：P01116-2
Sequences：M1-M188(end)
Molecular Weight：23.6 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The KRAS[G12D] activity was detected using HTRF technology. The reaction was performed by incubating the KRAS[G12D] protein, cRAF and the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
