cRAF
RAF proto-oncogene serine/threonine-protein kinase, RAF1, RAF
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Catalog Number:E2211T-H36G
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Description：Recombinant human cRAF protein with N-terminal GST tag was purified by GST affinity and followed by Cation Ion-exchange Separation. The cRAF protein showed high activity in HTRF assay.
Species：Human
Tag：N-terminal GST
Expression Host：HEP E.coli
Uniprot：P04049-1
Sequences：S52-S188
Molecular Weight：42.0 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>65%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The cRAF activity was detected using HTRF technology. The reaction was performed by incubating the cRAF protein, GTP, KRAS[G12D] and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
