Biotin-CypA
Peptidyl-prolyl cis-trans isomerase A, PPIA
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Catalog Number:E2605F-H03A
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COA:Please contact us
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Description：Recombinant human Biotin-CypA protein with N-terminal His-Avi tag was purified by Ni-NTA affinity and specifically biotinylated at Avi tag in vitro, the His tag was removed by TEV, followed by SEC chromatography. The Biotin-CypA protein showed high activity in HTRF assay.
Species：Human
Tag：N-terminal Avi tag
Expression Host：HEP E.coli
Uniprot：P62937-1
Sequences：M1-E165(end)
Molecular Weight：20 kDa
Formulation：50mM Tris, 150mM NaCl, pH7.5, 1mM TCEP, 10% Glycerol
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-CypA activity was quantified using a HTRF-based assay. The reaction was performed by incubating the Biotin-CypA protein, substrate and the donor and receptor, and keeping the mixture at 25℃ for 60 min, finally measuring the 665nm/620nm fluorescence signal ratio on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
