Biotin-NRAS[G12V]
GTPase NRas, NRAS1
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Catalog Number:E2601T-H06HA
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COA:Please contact us
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Description：Recombinant human Biotin-NRAS[G12V] protein with N-terminal 6x His tag and C-terminal Avi tag was specifically biotinylated at Avi tag in vivo and purified by Ni-NTA affinity. The Biotin-NRAS[G12V] protein showed high activity in HTRF assay.
Species：Human
Tag：N-terminal 6x His tag, C-terminal Avi tag
Expression Host：HEP E.coli
Uniprot：P01111
Sequences：M1-N172, G12V
Molecular Weight：23.6 kDa
Formulation：20mM Tris, 300mM NaCl, pH7.5, 1mM TCEP, 300mM imidazole, 10% Glycerol
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
![M160224011-Biotin-NRAS[G12V].png](https://protein.ice-biosci.com/uploads/20260827/0e985559f66ec6e7.png)
Activity Identification：The Biotin-NRAS[G12V] activity was quantified using a HTRF-based assay. The reaction was performed by incubating the Biotin-NRAS[G12V] protein, GTP and substrate at 25℃ for 60 min, then adding the donor and receptor, and keeping the mixture at 25℃ for 60 min, finally measuring the 665nm/620nm fluorescence signal ratio on BMG.
![M160224011-Biotin-NRAS[G12V].png-1.png](https://protein.ice-biosci.com/uploads/20260827/381f88bd9b15be2e.png)
![M160224011-Biotin-NRAS[G12V].png-2.png](https://protein.ice-biosci.com/uploads/20260827/cf934446d9ab65bd.png)
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
