Biotin-CypA
Peptidyl-prolyl cis-trans isomerase A, PPIA
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Catalog Number:E2505F-R08HA
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COA:Please contact us
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Price:Please contact us
Description：Recombinant rat Biotin-CypA protein with N-terminal His tag and C- terminal Avi tag was purified by Ni-NTA affinity and specifically biotinylated at Avi tag in vitro, followed by SEC chromatography. The Biotin-CypA protein showed high activity in nanoDSF assay.
Species：Rat
Tag：N-terminal His-TEV, C- terminal Avi tag
Expression Host：HEP E.coli
Uniprot：P10111
Sequences：M1-L164(end)
Molecular Weight：21.9 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-CypA activity was quantified using nanoDSF assay. The reaction was performed by incubating the Biotin-CypA protein and 1%DMSO at 25℃ for 15 min, and then on PrometheusTM NT.48 heating the reaction mixture with the temperature range from 20℃ to 95℃, and real time measuring 350/330 nm fluorescence signal ratio.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
