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WRN[C727A]

DNA helicase

Catalog Number:
S2507T-H02HS
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Description:Recombinant human WRN[C727A] protein with N-terminal His-Sumo tag was purified by Ni-NTA affinity and followed by desalting. The WRN[C727A] protein showed high activity in ADP-Glo assay.


Species:Human


Tag:N-terminal His-Sumo tag-TEV


Expression Host:HEP sf9


Uniprot:Q14191


Sequences:G519–S1245


Molecular Weight:96 kDa


Formulation:25 mM Tris, 310 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5


Form:Liquid


Purity:>70%


Stability&Storage:Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.


SDS-PAGE:

  G150822011-WRN[C727A].png


Activity Identification:The WRN[C727A] activity was quantified using the ADP-Glo™ assay. The reaction was performed by incubating the WRN[C727A] protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.

G150822011-WRN[C727A].png-1.pngG150822011-WRN[C727A].png-3png.pngG150822011-WRN[C727A].png-12.png

Note:

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.

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