POLQ
DNA polymerase theta, Chaos1
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Catalog Number:S2311T-M01H
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COA:Please contact us
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Price:Please contact us
Description：Recombinant Mouse POLQ protein with N-terminal 6x His tag was purified by Ni-NTA affinity and followed by desalting. The POLQ protein showed high activity in ADP-Glo assay.
Species：Mouse
Tag：N-terminal His tag
Expression Host：HEP sf9
Uniprot： Q8CGS6
Sequences：M1-M890
Molecular Weight：101.1 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The POLQ activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the POLQ protein, ATP and substrate at 25℃ for 60 min, and adding ADP-Glo reagent at 25℃ for 40 min, then reading RLU with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
