PARP10
Protein mono-ADP-ribosyltransferase PARP10
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Catalog Number:S2203T-H39FS
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COA:Please contact us
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Description：Recombinant human PARP10 protein with N-terminal Flag tag and C-terminal Strep II tag was purified by Strep II affinity and followed by SEC chromatography. The PARP10 protein showed high activity in ELISA assay.
Species：Human
Tag：N-terminal Flag tag, C-terminal Strep II tag
Expression Host：HEP sf9
Uniprot： Q53GL7
Sequences：G805-T1025 (end)
Molecular Weight：27.1 kDa
Formulation：20 mM Hepes, 300 mM NaCl, 1 mM DTT, pH7.0
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The PARP10 activity was assayed with ELISA. The reaction was performed by: 1). Histone protein was coated on a 96-well plate. 2).A biotinylated NAD+ mix is incubated with varying different concentration of the PARP10 enzyme and an activated DNA. 3).The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
