PARP1
Poly [ADP-ribose] polymerase 1, ADPRT,PPOL
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Catalog Number:S2202T-H27H
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human PARP1 protein with N-terminal 6x His tag was purified by Ni-NTA affinity and followed by SEC chromatography. The PARP1 protein showed high activity in ELISA assay.
Species：Human
Tag：N-terminal 6x His tag
Expression Host：HEP sf9
Uniprot： P09874
Sequences： A2-W1014 (end)
Molecular Weight：115.1 kD
Formulation：20 mM Hepes, 300 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The PARP1 activity was assayed with ELISA. The reaction was performed by: 1). Histone protein was coated on a 96-well plate. 2).A biotinylated NAD+ mix is incubated with varying different concentration of the PARP1 enzyme and an activated DNA. 3).The plate was treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence. Finally, measured the signal using a chemiluminescence reader.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
