推荐 POLQ-N
POLH
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Catalog Number:S2201T-H02H
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human POLQ-N protein with N-terminal 6x His tag was purified by Ni-NTA affinity and followed by SEC chromatography. The POLQ-N protein showed high activity in ADP-Glo assay.
Species：Human
Tag：N-terminal 6x His tag
Expression Host：HEP sf9
Uniprot： O75417-1
Sequences：M1-M894
Molecular Weight：101.8 kDa
Formulation：20 mM Tris, 500 mM NaCl, 2mM B-ME, 5% glycerol, pH 8.5
Form：Liquid
Purity：>90%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The POLQ-N activity was detected using ADP-Glo technology. The reaction was performed by incubating the POLQ-N protein, ATP and ssDNA at 25℃ for 40 min, then adding ADP-Glo reagent and reading RLU with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
