POLN
DNA polymerase nu
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Catalog Number:E2212T-H19H
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human POLN protein with N-terminal 6x His tag and C-terminal Flag tag by Ni-NTA affinity and followed by SEC chromatography. The POLN protein showed high activity in TR-FRET assay.
Species：Human
Tag：N-terminal 6x His tag, C-terminal Flag tag
Expression Host：HEP E.coli
Uniprot： Q7Z5Q5-1
Sequences：A192-G863
Molecular Weight：78.5 kDa
Formulation：20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The POLN activity was detected using TR-FRET technology. The reaction was performed by incubating varying concentration of Doxorubicin and the POLN protein at 25℃ for 10 min, adding the dNTP, dsDNA and the beads at 25℃ for 120 min, then reading ratio signal with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
