PARG
poly(ADP-ribose) glycohydrolase
-
Catalog Number:E2512F-R05H
-
Size：
-
COA:Please contact us
-
Price:Please contact us
Description：Recombinant rat PARG protein with C-terminal His tag was purified by Ni-NTA affinity and followed by SEC chromatography. The PARG protein showed high activity in HTRF-based assay..
Species：Rat
Tag：C-terminal His tag
Expression Host：HEP E.coli
Uniprot：Q9QYM2
Sequences：M1-T972(end)
Molecular Weight：110.9 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>80%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The PARG activity was quantified using an HTRF-based assay. The reaction was performed by incubating the PARG protein and substrate at 25℃ for 60 min, then adding the donor and receptor, and keeping the mixture at 25℃ for 60 min, finally measuring the 665nm/620nm fluorescence signal ratio on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
