Biotin-PIK3CA/PIK3R1
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Catalog Number:S2510F-R09HAF2
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Description：Recombinant rat Biotin-PIK3CA protein with N-terminal His-Avi tag was co-expressed with PIK3R1 with N-terminal Flag tag. Recombinant rat Biotin-PIK3CA/PIK3R1 protein was biotinylated in vivo, purified by Ni affinity and followed by SEC chromatograph.The Biotin-PIK3CA/PIK3R1 protein showed good activity in ADP-Glo assay.
Species：Rat
Tag：PIK3CA: N-terminal His-TEV-Avi; PIK3R1: N-terminal Flag
Expression Host：HEP sf9
Uniprot：PIK3CA: A0A0G2K344; PIK3R1: Q63787
Sequences：PIK3CA: M1-N1068(end); PIK3R1:M1-R724(end)
Molecular Weight：PIK3CA: 128.3 kDa; PIK3R1: 84.9 kDa
Formulation：50 mM Tris, pH 7.5, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% Glycerol
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-PIK3CA/PIK3R1 kinase activity was quantified using the ADP-Glo™ assay. The reaction was performed by incubating the Biotin-PIK3CA/PIK3R1 protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
