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推荐 Biotin-STAT6

Signal transducer and activator of transcription 6

Catalog Number:
E2506T-H15HA
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Description:Recombinant human Biotin-STAT6 protein with N-terminal His tag and C-terminal Avi tag was purified by Ni-NTA affinity and specifically biotinylated at Avi tag in vitro, followed by  SEC chromatography. The Biotin-STAT6 protein showed high activity in TR-FRET assay.


Species:Human


Tag:N-terminal His tag-TEV, C-terminal Avi tag


Expression Host:HEP E.coli


Uniprot:P42226-1


Sequences:W123-T658


Molecular Weight:63.9 kDa


Formulation:50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5


Form:Liquid


Purity:>90%


Stability&Storage:Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.


SDS-PAGE:

  M150813012-Biotin-STAT6.png


Activity Identification:The Biotin-STAT6 activity was quantified using TR-FRET-based assay. The reaction was performed by incubating the Biotin-STAT6 protein and substrate, the donor and receptor at 25℃ for 60 min, then measuring the 520nm/485nm fluorescence signal ratio on BMG.

M150813012-Biotin-STAT6.png-1.pngM150813012-Biotin-STAT6.png-2.png

Note:

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.

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