VAV1
Proto-oncogene vav
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Catalog Number:E2501T-R12G
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant rat VAV1 protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The VAV1 protein showed high activity in HTRF assay.
Species：Rat
Tag：N-terminal GST-tag-Thrombin
Expression Host：HEP E.coli
Uniprot：P54100
Sequences：K780-S840
Molecular Weight：33.5 kDa
Formulation：50mM Tris, 150mM NaCl, 1mM DTT, 10% Glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The VAV1 activity was quantified using a HTRF-based assay. The reaction was performed by incubating the VAV1 protein and CRBN, the donor and receptor at 25℃ for 60 min, then measuring the 665nm/620nm fluorescence signal ratio on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
