Biotin-PCSK9
NARC1
-
Catalog Number:H2409F-H10AH
-
Size：
-
COA:Please contact us
-
Price:Please contact us
Description：Recombinant human Biotin-PCSK9 protein with C-terminal Avi-His tag was purified by Ni affinity and biotinylated in vitro then followed by SEC chromatography. The Biotin-PCSK9 protein showed high activity in HTRF assay.
Species：Human
Tag：C-terminal Avi-TEV-His-tag
Expression Host：HEP 293
Uniprot：Q8NBP7-1
Sequences：Q31-Q292
Molecular Weight：78.1 kDa
Formulation：50mM Tris, 150mM NaCl, 1mM DTT, 10% Glycerol, pH7.5
Form：Liquid
Purity：>80%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-PCSK9 activity was quantified using a HTRF-based assay. The reaction was performed by incubating the Biotin-PCSK9 protein and substrate, the donor and receptor at 25℃ for 60 min, then measuring the 665nm/620nm fluorescence signal ratio on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
