Biotin-ANDR(DBD)
Androgen receptor, DHTR, NR3C4, AR
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Catalog Number:E2312T-H05HA
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human Biotin-ANDR(DBD) protein with N-terminal 6x His tag and C-terminal Avi tag was purified by Ni-NTA affinity and biotinylation and followed by SEC chromatography. The Biotin-ANDR(DBD) protein showed high activity in TR-FRET assay.
Species：Human
Tag：N-terminal 6x His tag, C-terminal Avi tag
Expression Host：HEP E.coli
Uniprot：P10275-1
Sequences：D537-E679
Molecular Weight：20.1 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>85%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-ANDR(DBD) activity was detected by TR-FRET binding assay. The reaction was performed by incubating the varing concentrations of tool compound and Biotin-ANDR(DBD) protein, dsDNA and adding the beads at 25℃ for 60 min and reading ratio 485/520 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
