YAP
Transcriptional coactivator YAP1, YAP65
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Catalog Number:E2301T-H38HSA
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human YAP protein with N-terminal 6x His tag and sumo tag and C-terminal Avi tag was purified by GST affinity and followed by SEC chromatography. The YAP protein showed good activity in HTRF assay.
Species：Human
Tag：N-terminal 6x His tag and sumo tag, C-terminal Avi tag
Expression Host：HEP E.coli
Uniprot：P46937
Sequences：A50-E100
Molecular Weight：21.1 kDa
Formulation：50 mM Tris, 300 mM NaCl, 10% glycerol, pH7.5
Form：Liquid
Purity：>75%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The YAP activity was detected using HTRF technology. The reaction was performed by incubating the YAP protein, TEAD4 and the beads at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
