Biotin-CD73
5'-nucleotidase, NT5E, NT5, NTE
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Catalog Number:H2307T-H08AH
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COA:Please contact us
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Description：Recombinant human Biotin-CD73 protein with C-terminal 6x His and Avi tag was purified by Ni-NTA affinity and biotinylation and followed by desalting. The Biotin-CD73 protein showed high activity in AMP-Glo assay.
Species：Human
Tag：C-terminal 6x His and Avi tag
Expression Host：HEP 293
Uniprot：P21589
Sequences：W27-K547
Molecular Weight：61.4 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification： The Biotin-CD73 activity was detected using AMP-Glo technology. The reaction was performed by incubating the Biotin-CD73 protein and AMP at 25℃ for 120 min, adding AMP-Glo reagent at 25℃ for 40 min, then reading RLU with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
