Biotin-PDE4B
Phosphodiesterase
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Catalog Number:S2601F-M15A
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COA:Please contact us
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Price:Please contact us
Description：Recombinant mouse Biotin-PDE4B protein with N-terminal-GST-TEV and C-terminal Avi tag was specifically biotinylated at Avi tag in vivo and purified by GST affinity, the GST tag was removed by TEV protease, then the protein was further purified by SEC chromatography. The Biotin-PDE4B protein showed good activity in FP assay.
Species：Mouse
Tag：C-terminal Avi tag
Expression Host：HEP sf9
Uniprot：B1AWD0
Sequences：K2-T564(end)
Molecular Weight：66.4 kDa
Formulation：50mM Tris, 150mM NaCl, pH7.5, 1mM TCEP, 10% Glycerol
Form：Liquid
Purity：>80%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-PDE4B activity was quantified using a FP-based assay. The reaction was performed by incubating the Biotin-PDE4B protein and cAMP-FAM at 25℃ for 60 min, then adding the binding reagent, finally measuring FP signal on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
