Biotin-STAT6(phosphorylation)
Signal transducer and activator of transcription 6
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Catalog Number:S2510T-H01A
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COA:Please contact us
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Description：Recombinant human Biotin-STAT6 (phosphorylation) protein with N-terminal His tag and C-terminal Avi tag was purified by Ni-NTA affinity and followed by SEC chromatography. The protein was activated by JAK1, biotinylated and enzymatically cleaved in vitro. Subsequently, GST affinity and SEC chromatography were performed. The Biotin-STAT6 (phosphorylation) protein showed good activity in TR-FRET assay.
Species：Human
Tag：C-terminal Avi tag
Expression Host：HEP sf9
Uniprot：P42226
Sequences：W123-T658
Molecular Weight：61.9 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-STAT6 (phosphorylation) activity was quantified using TR-FRET-based assay. The reaction was performed by incubating the Biotin-STAT6 (phosphorylation) protein and substrate, the donor and receptor at 25℃ for 60 min, then measuring the 520nm/485nm fluorescence signal ratio on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
