Biotin-HIF1α
Hypoxia-inducible factor 1-alpha
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Catalog Number:E2502T-H01AH
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human Biotin-HIF1α protein with N-terminal Avi-His tag was purified by Ni-NTA affinity and specifically biotinylated at Avi tag in vitro, followed by SEC chromatography. The Biotin-HIF1α protein showed high activity in special shift-based assay.
Species：Human
Tag：N-terminal Avi-His-TEV tag
Expression Host：HEP E.coli
Uniprot：Q16665-1
Sequences：S239-L350
Molecular Weight：17.2 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-HIF1α activity was quantified using special shift-based assay. The reaction was performed by incubating the Biotin-HIF1α protein labeled by dye and compound at 25℃ for 15min, then measuring 670/650nm fluorescence signal ratio on Dianthus.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
