Biotin-STUB1
E3 ubiquitin-protein ligase CHIP, CHIP
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Catalog Number:E2510T-H16HA
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human Biotin-STUB1 protein with N-terminal His-Avi tag was purified by Ni-NTA affinity and specifically biotinylated at Avi tag in vitro, followed by SEC chromatography. The Biotin-STUB1 protein showed high activity in TR-FRET assay.
Species：Human
Tag：N-terminal His-TEV- Avi tag
Expression Host：HEP E.coli
Uniprot：Q9UNE7-1
Sequences：S23-R154
Molecular Weight：19.1 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-STUB1 activity was quantified using TR-FRET-based assay. The reaction was performed by incubating the Biotin-STUB1 protein and substrate, the donor and receptor at 25℃ for 60 min, then measuring the 520nm/490 nm fluorescence signal ratio on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
