Biotin-TRIM21
E3 ubiquitin-protein ligase TRIM21, RNF81, RO52, SSA1
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Catalog Number:S2407F-H02HA
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human Biotin-TRIM21 protein with C-terminal Avi-His tag was specifically biotinylated at Avi tag in vivo.The Biotin-TRIM21 protein was purified by Ni affinity chromatography. The Biotin-TRIM21 protein showed high activity in nanoDSF assay.
Species：Human
Tag：C-terminal Avi-TEV-His tag
Expression Host：HEP sf9
Uniprot：P19474-1
Sequences：M1-Y475(end)
Molecular Weight：57.6 kDa
Formulation：20 mM Tris, 300 mM NaCl, 300 mM imidazole, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>70%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-TRIM21 activity was quantified using nanoDSF assay. The reaction was performed by incubating the Biotin-TRIM21 protein at 25℃ for 15 min, and then on PrometheusTM NT.48 heating the reaction mixture with the temperature range from 20℃ to 95℃, and real time measuring 350/330 nm fluorescence signal ratio.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
