Biotin-STAT6[Y616W]
Signal transducer and activator of transcription 6
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Catalog Number:E2507T-H01HA
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Description：Recombinant human Biotin-STAT6[Y616W] protein with N-terminal His tag and C-terminal Avi tag was purified by Ni-NTA affinity and specifically biotinylated at Avi tag in vitro, followed by GST affinity and SEC chromatography. The Biotin-STAT6[Y616W] protein showed high activity in SPR assay.
Species：Human
Tag：N-terminal His tag-TEV, C-terminal Avi tag
Expression Host：HEP E.coli
Uniprot：P42226-1
Sequences：W123-T658, Y616W
Molecular Weight：63.9 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
![K150827011-Biotin-STAT6[Y616W].png](/uploads/20251128/f9ec19a2cb2fe380.png)
Activity Identification：The Biotin-STAT6[Y616W] activity was quantified using using a SPR-based assay. The Biotin-STAT6[Y616W] protein was immobilized onto the CM5 chip. The varying concentration of STAT6-IN-3 was injected, and flowed over onto the Biotin-STAT6[Y616W] protein surface. And the response signal was recorded real time. The kinetics fitting was as follows with a KD value of ~ 2.54x10-8 M.
![K150827011-Biotin-STAT6[Y616W].png-1.png](/uploads/20251128/de0ba333e59e870f.png)
![K150827011-Biotin-STAT6[Y616W].png-2.png](/uploads/20251128/48fb69f4650825a5.png)
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
