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CDK1/CycA2

Cyclin-dependent kinase 1/CyclinA2

Catalog Number:
S2211F-H58GH2
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Description:Recombinant human CDK1 with N-terminal GST tag was co-expressed with CycA2 protein with N-terminal GST tag and C-terminal 8x His tag. Recombinant human CDK1/CycA2 protein was purified by GST affinity and followed by SEC chromatography. The CDK1/CycA2 protein showed high activity in ADP-Glo assay.


Species:Human


Tag:CDK1: N-terminal GST tag; CycA2: N-terminal GST tag, C-terminal 8x His tag


Expression Host:HEP sf9


Uniprot:CDK1: P06493-1; CycA2: P20248


Sequences:CDK1: M1-M297(end); CycA2: E174-L432(end)


Molecular Weight:CDK1: 60.7 kDa; CycA2: 56.8 kDa


Formulation:50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5


Form:Liquid


Purity:>95%


Stability & Storage:Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.


SDS-PAGE:

SDS-1.png


Activity Identification:The CDK1/CycA2 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK1/CycA2 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.

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Note:

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.

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