Biotin-ERK2
Mitogen-activated protein kinase 1, MAPK1, PRKM1, PRKM2
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Catalog Number:S2404F-H22HA
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COA:Please contact us
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Description：Recombinant human Biotin-ERK2 protein with N-terminal 6x His tag and Avi tag was performed in vivo biotinylation, and purified by Ni-NTA affinity, and then performed in vitro activation by recombinant human MAP2K1 protein, and purified by SEC chromatography. The Biotin-ERK2 protein showed high activity in ADP-Glo assay.
Species：Human
Tag：N-terminal 6x His and Avi tag
Expression Host：HEP sf9
Uniprot：P28482-1
Sequences：A2-S360(end)
Molecular Weight：45.3 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% Glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-ERK2 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the Biotin-ERK2 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
