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CDK9/CyclinT2

Cyclin-dependent kinase 9/CyclinT2

Catalog Number:
S2401F-H05GHF2
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Description:Recombinant human CDK9 with N-terminal GST tag was co-expressed with CyclinT2 protein with N-terminal Flag and 8x His Tag. Recombinant human CDK9/CyclinT2 protein was purified by Flag affinity and followed by desalting. The CDK9/CyclinT2 protein showed high activity in ADP-Glo assay.


Species:Human


Tag:CDK9: N-terminal GST tag; CyclinT2: N-terminal Flag and 8x His tag


Expression Host:HEP sf9


Uniprot: CDK9: P50750; CyclinT2: O60583-2


Sequences:CDK9: M1-F372(end); CyclinT2: A2-S663(end)


Molecular Weight:CDK9: 68.6 kDa; CyclinT2: 77.0 kDa


Formulation:50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5


Form:Liquid


Purity:>90%


Stability & Storage:Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.


SDS-PAGE:

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Activity Identification:The CDK9/CyclinT2 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK9/CyclinT2 protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.

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2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.

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