CDK4/CycD3[S259A]
Cyclin-dependent kinase 4/CyclinD3[S259A]
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Catalog Number:S2305F-H43GH2
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Description：Recombinant human CDK4 with N-terminal GST tag was co-expressed with CycD3[S259A] protein with C- terminal 6x His Tag. Recombinant human CDK4/CycD3[S259A] protein was purified by Ni-NTA affinity. The CDK4/CycD3[S259A] protein showed high activity in ADP-Glo assay.
Species：Human
Tag：CDK4: N-terminal GST tag; CycD3[S259A]: C-terminal 6x His tag
Expression Host：HEP sf9
Uniprot： CDK4: P11802-1; CycD3: P30281-1
Sequences： CDK4: M1-E303(end); CycD3: M1-L292(end), S259A
Molecular Weight：CDK4: 59.6 kDa; CycD3[S259A]: 33.5 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The CDK4/CycD3[S259A] activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the CDK4/CycD3[S259A] protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
