CDK7/CCNH/MNAT1
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Catalog Number:S2606F-H05FG3
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COA:Please contact us
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Description：Recombinant human CDK7 protein was co-expressed with CCNH protein carrying C-terminal Flag and MNAT1 protein bearing N-terminal GST-TEV tag. The resulting CDK7/CCNH/MNAT1 ternary complex was purified by GST affinity, followed by SEC chromatography, and exhibited robust activity in the ADP‑Glo assay.
Species：Human
Tag：CDK7: No tag; CCNH: C-terminal Flag; MNAT1: N-terminal GST-TEV tag
Expression Host：HEP sf9
Uniprot：CDK7: P50613; CCNH: P51946; MNAT1: P51948
Sequences：CDK7: A2-F346; CCNH: Y2-L323; MNAT1: D2-S309
Molecular Weight：CDK7: 39.1 kDa; CCNH: 38.8 kDa; MNAT1: 62.3 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
Form：Liquid
Purity：>80%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The CDK7/CCNH/MNAT1 kinase activity was quantified using the ADP-Glo assay. The reaction was performed by incubating the CDK7/CCNH/MNAT1 protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
