CDK13/Cyclin T1
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Catalog Number:S2504T-H13GH2
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Description：Recombinant human CDK13 with N-terminal GST tag was co-expressed with Cyclin T1 protein with N-terminal GST and C-terminal His Tag. Recombinant human CDK13/Cyclin T1 protein was purified by GST affinity. The CDK13/Cyclin T1 protein showed high activity in HTRF assay.
Species：Human
Tag：CDK13: N-terminal GST tag; Cyclin T1: N-terminal-GST, C-terminal His tag
Expression Host：HEP sf9
Uniprot：CDK13: Q14004; Cyclin T1: O60563-1
Sequences：CDK13: Q673-S1054; Cyclin T1: M1-K726(end)
Molecular Weight：CDK13: 70.3 kDa; Cyclin T1: 107.8 kDa
Formulation：50 mM Tris, 150 mM NaCl, 5 mM GSH,1 mM DTT, 0.05% Brij35, 10% glycerol, pH 7.5
Form：Liquid
Purity：>60%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The CDK13/Cyclin T1 activity was quantified using a HTRF-based assay. The reaction was performed by incubating the CDK13/Cyclin T1 protein, ATP and substrate at 25℃ for 60 min, then adding the donor and receptor, and keeping the mixture at 25℃ for 60 min, finally measuring the 665nm/620nm fluorescence signal ratio on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
