BRAF
Serine/threonine-protein kinase B-raf, BRAF1, RAFB1
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Catalog Number:S2302T-H20G
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human BRAF protein with N-terminal GST tag and C-terminal 6x His tag was purified by Ni-NTA affinity and followed by GST affinity chromatography. The BRAF protein showed high activity in ADP-Glo assay.
Species：Human
Tag：N-terminal GST tag, C-terminal 6x His tag
Expression Host：HEP sf9
Uniprot：P15056
Sequences：E433-R726
Molecular Weight：61.3 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>80%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The BRAF activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
