Biotin-RIPK2
Receptor-interacting serine/threonine-protein kinase 2, RICK 1, RIP2
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Catalog Number:S2601T-H13HA
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Description：Recombinant human Biotin-RIPK2 protein with N-terminal His Avi tag was biotinylated in vivo, purified by Ni affinity and followed by SEC chromatograph. The Biotin-RIPK2 protein showed high activity in SPR assay.
Species：Human
Tag：N-terminal His-TEV-Avi tag
Expression Host：HEP sf9
Uniprot：O43353-1
Sequences：N2-A305
Molecular Weight：38.9 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM TCEP, 0.05% Brij-35, 10% glycerol, pH 7.5
Form：Liquid
Purity：>80%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-RIPK2 activity was quantified using an SPR-based assay. The Biotin-RIPK2 protein was immobilized onto the SA chip. The varying concentration of WEHI-345 was injected, and flowed over onto the Biotin-RIPK2 protein surface. And the response signal was recorded in real time. The kinetics fitting was as follows with a kD value of ~ 2.45x10-8 M.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
