Biotin-LYN
Tyrosine-protein kinase Lyn, JTK8
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Catalog Number:S2411F-H09FA
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Size：
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COA:Please contact us
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Price:Please contact us
Description：Recombinant human Biotin-LYN protein with N-terminal Flag-Avi tag was purified by Flag affinity SEC chromatography. The Biotin-LYN protein showed high activity in HTRF assay.
Species：Human
Tag：N-terminal Flag-Avi tag
Expression Host：HEP sf9
Uniprot：P07948
Sequences：G2-P512(end)
Molecular Weight：61.5 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35,10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-LYN activity was quantified using a HTRF-based assay. The reaction was performed by incubating the Biotin-LYN protein, ATP and substrate at 25℃ for 60 min, then adding the donor and receptor, and keeping the mixture at 25℃ for 60 min, finally measuring the 665nm/620nm fluorescence signal ratio on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
