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Biotin-JAK1[JH2]

JAK1A, JAK1B

Catalog Number:
S2501T-H05HA
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Description:Recombinant human Biotin-JAK1[JH2] protein with N-terminal His tag and C-terminal Avi was expressed with bira for  biotinylation in vivo, purified by Ni-NTA affinity chromatography. The Biotin-JAK1[JH2] protein showed high activity in FP assay.


Species:Human


Tag:N-terminal -His, C-terminal Avi


Expression Host:HEP sf9


Uniprot:P23458


Sequences:A561-N852


Molecular Weight:36.8 kDa


Formulation:50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5


Form:Liquid


Purity:>90%


Stability&Storage:Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.


SDS-PAGE:

  K150311011-Biotin-JAK1[JH2].png


Activity Identification:The Biotin-JAK1[JH2] activity was quantified using a FP-based assay. The reaction was performed by incubating the Biotin-JAK1[JH2] protein, ATP and FAM-Cyclic-3,5-AMP at 25℃ for 60 min, then adding the binding reagent, finally measuring FP signal on BMG.

K150311011-Biotin-JAK1[JH2].png-1.png

Note:

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.

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