BTK[T474S]
Tyrosine-protein kinase BTK, AGMX1, ATK, BPK
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Catalog Number:S2301T-H04G
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Description：Recombinant human BTK[T474S] protein with N-terminal GST tag was purified by GST affinity followed by SEC chromatography. The BTK[T474S] protein showed high activity in HTRF assay.
Species：Human
Tag：N-terminal GST tag
Expression Host：HEP sf9
Uniprot：Q06187-1
Sequences： A2-S659(end), T474S
Molecular Weight：101.9 kDa
Formulation：20 mM Hepes, 300 mM NaCl, 1 mM DTT, 0.1 mM PMSF, 10% glycerol, 0.05% Brij35, pH7.5
Form：Liquid
Purity：>90%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The BTK[T474S] activity was detected using HTRF technology. The reaction was performed by incubating the BTK[T474S] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
