EGFR[D746-750/T790M/C797G]

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Epidermal growth factor receptor, ERBB, ERBB1, HER1

Catalog Number:

S2509T-H05F

Size：

10 μg 100 μg 1 mg

COA:

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Product Information
Routine QC
Activity Identification

Description：Recombinant human EGFR[d746-750/T790M/C797G] protein with N-terminal Flag tag was purified by Flag affinity chromatography and followed by desalting. The EGFR[d746-750/T790M/C797G] protein showed high activity in HTRF assay.

Species：Human

Tag：N-terminal Flag tag

Expression Host：HEP sf9

Uniprot：P00533-1

Sequences：G696-G1022, d746-750, T790M, C797G

Molecular Weight：37.9 kDa

Formulation：50 mM Tris, 300 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5

Form：Liquid

Purity：>70%

Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.

SDS-PAGE：

G151211021-EGFR[d746-750 T790M C797G].pn

Activity Identification：The EGFR[d746-750/T790M/C797G] activity was quantified using a HTRF-based assay. The reaction was performed by incubating the EGFR[D746-750/T790M/C797G] protein, ATP and substrate at 25℃ for 60 min, then adding the donor and receptor, and keeping the mixture at 25℃ for 60 min, finally measuring the 665nm/620nm fluorescence signal ratio on BMG.

G151211021-EGFR[d746-750 T790M C797G].pn

Note：

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.