Biotin-JAK2[JH2][W659A, W777A, F794H]

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Tyrosine-protein kinase JAK2

Catalog Number:

S2501T-H07HA

Size：

10 μg 100 μg 1 mg

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Product Information
Routine QC
Activity Identification

Description：Recombinant human Biotin-JAK2[JH2] [W659A,W777A,F794H] protein with C-terminal His-Avi was biotinylated in vivo, purified by Ni affinity and followed by SEC chromatography. The Biotin-JAK2[JH2] [W659A,W777A,F794H] protein showed high activity in TR-FRET-based assay.

Species：Human

Tag：C-terminal His-TEV-Avi

Expression Host：HEP sf9

Uniprot：O60674

Sequences：V536-D812, W659A,W777A,F794H

Molecular Weight：35.6 kDa

Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Form：Liquid

Purity：>60%

Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.

SDS-PAGE：

G150314012-Biotin-JAK2[JH2][W659A, W777A

Activity Identification：The Biotin-JAK2[JH2][W659A, W777A, F794H] activity was quantified using TR-FRET-based assay. The reaction was performed by incubating the Biotin-JAK2[JH2][W659A, W777A, F794H] protein and substrate, the donor and receptor at 25℃ for 60 min, then measuring the 520nm/485nm fluorescence signal ratio on BMG.

G150314012-Biotin-JAK2[JH2][W659A, W777A

Note：

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.