Biotin-PIK3CG
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
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Catalog Number:S2503F-H09HA
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Description：Recombinant human Biotin-PIK3CG protein with N-terminal His-Avi tag was purified by Ni-NTA affinity and followed by SEC chromatography. The Biotin-PIK3CG protein showed high activity in ADP-Glo assay.
Species：Human
Tag：N-terminal His tag-TEV-Avi
Expression Host：HEP sf9
Uniprot：P48736
Sequences：E2-A1102(end)
Molecular Weight：130.5 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35,10% glycerol, pH7.5
Form：Liquid
Purity：>60%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-PIK3CG activity was activity was quantified using the ADP-Glo™ assay. The reaction was performed by incubating the Biotin-PIK3CG protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
