Description：Recombinant human PIK3CA[H1047R] with C-terminal 8x His tag was co-expressed with PIK3R1 protein with N-terminal Flag tag and C-terminal Avi tag. Recombinant human Biotin-PIK3CA[H1047R]/PIK3R1 protein was purified by Ni-NTA affinity and followed by SEC chromatography. The Biotin-PIK3CA[H1047R]/PIK3R1 protein showed high activity in in SPR binding assay with small molecular.

Species：Human

Tag：PIK3CA[H1047R]: C-terminal 8x His tag; PIK3R1: N-terminal Flag tag, C-terminal Avi tag

Expression Host：HEP sf9

Uniprot：PIK3CA[H1047R]: P42336; PIK3R1: P27986

Sequences：PIK3CA[H1047R]: M1-N1068(end) [H1047R]; PIK3R1: M1-R724 (end)

Molecular Weight：PIK3CA[H1047R]: 125.5 kDa; PIK3R1: 86.8 kDa

Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Form：Liquid

Purity：>90%

Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.

SDS-PAGE：

Activity Identification：The Biotin-PIK3CA[H1047R]/PIK3R1 activity was detected using SPR technology. The Biotin-PIK3CA[H1047R]/PIK3R1 protein was immobilized onto the SA chip coupled with neutravidin using labeled biotin. The varying concentration of Alpelisib was injected onto the Biotin-PIK3CA[H1047R]/PIK3R1 protein surface, and recorded the response signal. The kinetics fitting was as follows with a kD value of ~ 2.05x10-9 M.