Biotin-PIK3CA[E545K]/PIK3R1
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
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Catalog Number:S2310F-H07HF2
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Description：Recombinant human PIK3CA[E545K] with C-terminal 8x His tag was co-expressed with PIK3R1 protein with N-terminal Flag tag and C-terminal Avi tag. Recombinant human Biotin-PIK3CA[E545K]/PIK3R1 protein was purified by Ni-NTA affinity and biotinylation and followed by SEC chromatography. The Biotin-PIK3CA[E545K]/PIK3R1 protein showed high activity in ADP-Glo assay.
Species：Human
Tag：PIK3CA[E545K]: C-terminal 8x His tag; PIK3R1: N-terminal Flag tag, C-terminal Avi tag
Expression Host：HEP sf9
Uniprot：PIK3CA: P42336; PIK3R1: P27986-1
Sequences：PIK3CA: M1-N1068(end), [E545K]; PIK3R1: M1-R724(end)
Molecular Weight：PIK3CA[E545K]: 125.5 kDa; PIK3R1: 86.8 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>95%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
![K140221021-Biotin-PIK3CA[E545K]-PIK3R1.p](/uploads/20240727/ce7d717fc3d13cfd.png)
Activity Identification：The Biotin-PIK3CA[E545K]/PIK3R1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the Biotin-PIK3CA[E545K]/PIK3R1 protein and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
![K140221021-Biotin-PIK3CA[E545K]-PIK3R1-1](/uploads/20240729/609e60f38b7560cc.png)
![K140221021-Biotin-PIK3CA[E545K]-PIK3R1-2](/uploads/20240729/e954bbc197e009d3.png)
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
