Biotin-LATS1/MOBKL1A
Serine/threonine-protein kinase LATS1, WARTS
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Catalog Number:S2309T-H35FAH2
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Description：Recombinant human LATS1 with N-terminal Flag tag and C-terminal Avi tag was co-expressed with MOBKL1A protein with N-terminal 6x His tag. Recombinant human Biotin-LATS1/MOBKL1A protein was purified by flag affinity and biotinylation and followed by desalting. The Biotin-LATS1/MOBKL1A protein showed high activity in ADP-Glo assay.
Species：Human
Tag：LATS1: N-terminal Flag tag, C-terminal Avi tag; MOBKL1A: N-terminal 6x His tag
Expression Host：HEP sf9
Uniprot：LATS1: O95835-1; MOBKL1A: Q7L9L4-1
Sequences：LATS1: K589-V1130(end); MOBKL1A: M1-R216(end)
Molecular Weight：LATS1: 66.5 kDa; MOBKL1A: 26.2 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5
Form：Liquid
Purity：>90%
Stability & Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-LATS1/MOBKL1A activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the Biotin-LATS1/MOBKL1A protein and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
