PRKD3
Serine/threonine-protein kinase D3,EPK2, PRKCN
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Catalog Number:S2405F-H46G
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Description：Recombinant human PRKD3 protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. SEC-HPLC result showed that the PRKD3 protein was dimer in the solution. The PRKD3 protein showed high activity in HTRF assay
Species：Human
Tag：N-terminal GST tag-3C
Expression Host：HEP sf9
Uniprot：O94806
Sequences：S2-P890(end)
Molecular Weight：127.2 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The PRKD3 activity was quantified using a HTRF-based assay. The reaction was performed by incubating the PRKD3 protein, ATP and substrate at 25℃ for 60 min, then adding the donor and receptor, and keeping the mixture at 25℃ for 60 min, finally measuring the 665nm/620nm fluorescence signal ratio on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
