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PIM1

Serine/threonine-protein kinase pim-1

Catalog Number:
S2305F-H58GH
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Description:Recombinant human PIM1 protein with N-terminal GST tag and C-terminal 8x His tag was purified by GST affinity and followed by SEC chromatogaphy. The PIM1 protein showed high activity in ADP-Glo assay.


Species:Human


Tag:N-terminal GST tag, C-terminal 8x His tag


Expression Host:HEP sf9


Uniprot:P11309-1


Sequences:M1-K313(end)


Molecular Weight:62.8 kDa


Formulation:50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5


Form:Liquid


Purity:>95%


Stability & Storage:Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.


SDS-PAGE:

  G130809012-PIM1.png


Activity Identification:The PIM1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the PIM1 protein, ATP and substrate at 25℃ for 60 min, and adding ADP-Glo reagent at 25℃ for 40 min, then reading RLU with BMG.

G130809012-PIM1-1.pngG130809012-PIM1-2.png


Note:

1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.

2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.

3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.

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