AMPKα1/β1/γ1(nonactivated)
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Catalog Number:S2404F-H64G3
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Description：Recombinant human AMPKα1/β1/γ1(nonactivated) is a ternary complex, which consists of PRKAA1(N-terminal GST tag), PRKAB1(N-terminal GST tag) and PRKAG1 (no tag).The AMPKα1/β1/γ1(nonactivated) protein was purified by GST affinity and followed by SEC chromatography. The AMPKα1/β1/γ1 protein showed high activity in ADP-Glo assay.
Species：Human
Tag：PRKAA1:N-terminal GST tag; PRKAB1:N-terminal GST tag; and PRKAG1:no tag
Expression Host：HEP sf9
Uniprot：PRKAA1: Q13131PRKAB1: Q9Y478; PRKAG1: P54619-1
Sequences：PRKAA1: M1-Q559 (end); PRKAB1: M1-I270(end); PRKAG1:M1-P331(end)
Molecular Weight：PRKAA1: 90.7 kDa; PRKAB1: 57.1 kDa; PRKAG1: 37.6 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 0.05% Brij35,10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The AMPKα1/β1/γ1 (non-activated) activity was quantified using the ADP-Glo™ assay. First, the AMPKα1/β1/γ1 (non-activated) protein was incubated with the CAMKK2 protein at 25°C for 30 minutes. ATP and substrate were then added, followed by incubation at 25°C for 60 minutes. Finally, the ADP-Glo reagent was added, and the luminescence signal was measured on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
