MELK
Maternal embryonic leucine zipper kinase
-
Catalog Number:S2503T-H01G
-
Size：
-
COA:Please contact us
-
Price:Please contact us
Description：Recombinant human MELK protein with N-terminal GST tag was purified by GST affinity chromatography. The MELK protein showed high activity in ADP-Glo™ assay.
Species：Human
Tag：N-terminal GST tag-TEV
Expression Host：HEP sf9
Uniprot：Q14680-1
Sequences：K2-M493
Molecular Weight：83 kDa
Formulation：50 mM Tris, 150 mM NaCl, 5 mM GSH, 1 mM DTT, 0.05% Brij35,10% glycerol, pH7.5
Form：Liquid
Purity：>80%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The MELK activity was quantified using the ADP-Glo™ assay. The reaction was performed by incubating the MELK protein, ATP and substrate at 25℃ for 60 min, adding ADP-Glo reagent and then measuring luminescence signal on BMG.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
