Biotin-MAP3K19
Mitogen-activated protein kinase kinase kinase 19, RCK, YSK4
-
Catalog Number:S2412T-H06FA
-
Size：
-
COA:Please contact us
-
Price:Please contact us
Description：Recombinant human Biotin-MAP3K19 protein with N-terminal Flag-Avi tag was performed in vivo biotinylation simultaneously. Recombinant human Biotin-MAP3K19 was purified by Flag affinity chromatography. The MAP3K19 protein showed high activity in SPR assay.
Species：Human
Tag：N-terminal Flag-Avi tag
Expression Host：HEP sf9
Uniprot：Q56UN5-1
Sequences：M941-H1328 (end)
Molecular Weight：46.7 kDa
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
Form：Liquid
Purity：>90%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
Activity Identification：The Biotin-MAP3K19 activity was quantified using a SPR-based assay. The Biotin-MAP3K19 protein was immobilized onto the SA chip. The varying concentration of staurosporine was injected, and flowed over onto the Biotin-MAP3K19 protein surface. And the response signal was recorded real time. The kinetics fitting was as follows with a kD value of ~ 2.26x10-9 M.
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
