MAP2K1[S218E/S222E]
Dual specificity mitogen-activated protein kinase kinase 1, MEK1, PRKMK1
-
Catalog Number:S2507T-H01G
-
Size：
-
COA:Please contact us
-
Price:Please contact us
Description：Recombinant human MAP2K1[S218E/S222E] protein with N-terminal GST tag was co-expressed with RAF1[Y340D/Y341D] protein with His tag. The MAP2K1[S218E/S222E] protein was purified by GST affinity and followed by Ni-NTA affinity chromatography. The MAP2K1[S218E/S222E] protein showed high activity in ADP-Glo assay.
Species：Human
Tag：N-terminal GST tag
Expression Host：HEP sf9
Uniprot：Q02750-1
Sequences：M1-V393(end) [S218E/S222E]
Molecular Weight：70.2 kDa
Formulation：25 mM Tris, 300 mM NaCl, 1 mM DTT, 0.05% Brij35, 10% Glycerol, pH7.5
Form：Liquid
Purity：>85%
Stability&Storage：Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
SDS-PAGE：
![G151224011-MAP2K1[S218E S222E].png](https://protein.ice-biosci.com/uploads/20260528/89f3b90a9fd9beed.png)
Activity Identification：The MAP2K1[S218E/S222E] activity was detected using ADP-Glo assay. The reaction was performed by incubating the MAP2K1[S218E/S222E] protein, ATP and substrate at 25℃ for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
![G151224011-MAP2K1[S218E S222E].png-1.png](https://protein.ice-biosci.com/uploads/20260528/3b0a04d4fdc9703b.png)
![G151224011-MAP2K1[S218E S222E].png-2.png](https://protein.ice-biosci.com/uploads/20260528/77a2ff4db0f6c6e1.png)
Note：
1. This product is for research use only. Do not use for medical, clinical diagnosis or treatment, food, or cosmetic purposes. Do not store in residential areas.
2. For your safety and health, please wear lab coats and disposable gloves and masks when handling.
3. Experimental results may be influenced by various factors. Related handling is limited to the product itself and does not involve other compensations.
